› 6udb

X-ray diffraction
1.55Å resolution

Spectroscopic and structural characterization of a genetically encoded direct sensor for protein-ligand interactions

Released:
DOI: 10.2210/pdb6udb/pdb
PDB entry 6udb coloured by chain, front view.
PDB entry 6udb coloured by chain, side view.
PDB entry 6udb coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Structural Origins of Altered Spectroscopic Properties upon Ligand Binding in Proteins Containing a Fluorescent Noncanonical Amino Acid.
Gleason PR, Kolbaba-Kartchner B, Henderson JN, Stahl EP, Simmons CR, Mills JH
Biochemistry 60 2577-2585 (2021)
PMID: 34415744

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 136 amino acids
Theoretical weight: 14.62 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family

Ligands and Environments

1 bound ligand:
Ligand PEG 4 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 46.458Å b: 85.555Å c: 58.098Å
α: 90° β: 99° γ: 90°
R-values:
R R work R free
0.167 0.166 0.201
Expression system: Escherichia coli

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Citations

1 review citation

Umbelliferone and Its Synthetic Derivatives as Suitable Molecules for the Development of Agents with Biological Activities: A Review of Their Pharmacological and Therapeutic Potential.
Kornicka et al. (2023)
2 other articles