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Structural basis for tRNA decoding and aminoacylation sensing by T-box riboregulators.

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Battaglia RA, Grigg JC, Ke A
OpenAccess logo Nat Struct Mol Biol 26 1106-1113 (2019)
Cited: 20 times
EuropePMC logo PMID: 31740853

Abstract

T-box riboregulators are a class of cis-regulatory RNAs that govern the bacterial response to amino acid starvation by binding, decoding and reading the aminoacylation status of specific transfer RNAs. Here we provide a high-resolution crystal structure of a full-length T-box from Mycobacterium tuberculosis that explains tRNA decoding and aminoacylation sensing by this riboregulator. Overall, the T-box consists of decoding and aminoacylation sensing modules bridged by a rigid pseudoknot structure formed by the mid-region domains. Stem-I and the Stem-II S-turn assemble a claw-like decoding module, while the antiterminator, Stem-III, and the adjacent linker form a tightly interwoven aminoacylation sensing module. The uncharged tRNA is selectively recognized by an unexpected set of favorable contacts from the linker region in the aminoacylation sensing module. A complex structure with a charged tRNA mimic shows that the extra moiety dislodges the linker, which is indicative of the possible chain of events that lead to alternative base-pairing and altered expression output.

Articles - 6ufh mentioned but not cited (1)

  1. Structural basis for tRNA decoding and aminoacylation sensing by T-box riboregulators. Battaglia RA, Grigg JC, Ke A. Nat Struct Mol Biol 26 1106-1113 (2019)


Reviews citing this publication (7)

  1. Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions. Zhang J. Wiley Interdiscip Rev RNA 11 e1600 (2020)
  2. Structural Insights into RNA Dimerization: Motifs, Interfaces and Functions. Bou-Nader C, Zhang J. Molecules 25 E2881 (2020)
  3. Cooperativity and Interdependency between RNA Structure and RNA-RNA Interactions. Skeparnias I, Zhang J. Noncoding RNA 7 81 (2021)
  4. The diverse structural modes of tRNA binding and recognition. Biela A, Hammermeister A, Kaczmarczyk I, Walczak M, Koziej L, Lin TY, Glatt S. J Biol Chem 299 104966 (2023)
  5. Engineering the Translational Machinery for Biotechnology Applications. Wang T, Liang C, An Y, Xiao S, Xu H, Zheng M, Liu L, Wang G, Nie L. Mol Biotechnol 62 219-227 (2020)
  6. Incorporation of nonstandard amino acids into proteins: principles and applications. Wang T, Liang C, Xu H, An Y, Xiao S, Zheng M, Liu L, Nie L. World J Microbiol Biotechnol 36 60 (2020)
  7. Recognition of the tRNA structure: Everything everywhere but not all at once. Zhang J. Cell Chem Biol 31 36-52 (2024)

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