› 6uiz

X-ray diffraction
1.85Å resolution

Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases

Released:
DOI: 10.2210/pdb6uiz/pdb
PDB entry 6uiz coloured by chain, front view.
PDB entry 6uiz coloured by chain, side view.
PDB entry 6uiz coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Artificial Iron Proteins: Modeling the Active Sites in Non-Heme Dioxygenases.
Miller KR, Paretsky JD, Follmer AH, Heinisch T, Mittra K, Gul S, Kim IS, Fuller FD, Batyuk A, Sutherlin KD, Brewster AS, Bhowmick A, Sauter NK, Kern J, Yano J, Green MT, Ward TR, Borovik AS
Inorg Chem 59 6000-6009 (2020)
PMID: 32309932

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 16.55 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 38-183; Coverage: 92%)
Sequence domains: Avidin family

Ligands and Environments

2 bound ligands:
Ligand QG4 1 x QG4
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I4122
Unit cell:
a: 57.75Å b: 57.75Å c: 184.499Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.234
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Chemical modifications of proteins and their applications in metalloenzyme studies.
Naowarojna et al. (2021)
4 more

1 mention without citation

Artificial Iron Proteins: Modeling the Active Sites in Non-Heme Dioxygenases.
Miller et al. (2020)