› 6uly

X-ray diffraction
2.3Å resolution

Adenylation domain of a keto acid-selecting NRPS module bound to keto acyl adenylate space group P212121

Released:
DOI: 10.2210/pdb6uly/pdb
PDB entry 6uly coloured by chain, front view.
PDB entry 6uly coloured by chain, side view.
PDB entry 6uly coloured by chain, top view.
Source organism: Bacillus stratosphericus LAMA 585
Primary publication:
Structural basis of keto acid utilization in nonribosomal depsipeptide synthesis.
Alonzo DA, Chiche-Lapierre C, Tarry MJ, Wang J, Schmeing TM
Nat Chem Biol 16 493-496 (2020)
PMID: 32066969

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241519 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amino acid adenylation domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 576 amino acids
Theoretical weight: 63.94 KDa
Source organism: Bacillus stratosphericus LAMA 585
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand QA7 2 x QA7
Ligand SO4 21 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 99.906Å b: 107.031Å c: 168.042Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Nonribosomal peptide synthetases and their biotechnological potential in Penicillium rubens.
Iacovelli et al. (2021)
2 more

2 mentions without citation

Biosynthesis of depsipeptides, or Depsi: The peptides with varied generations.
Alonzo et al. (2020)
1 more