› 6un6

X-ray diffraction
1.5Å resolution

Crystal structure of green fluorescent protein (GFP); S65T, Y66(3-NO2Y); ih circular permutant (50-51)

Released:
DOI: 10.2210/pdb6un6/pdb
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PDB entry 6un6 coloured by chain, front view.
PDB entry 6un6 coloured by chain, side view.
PDB entry 6un6 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Unusual Spectroscopic and Electric Field Sensitivity of Chromophores with Short Hydrogen Bonds: GFP and PYP as Model Systems.
Lin CY, Boxer SG
J Phys Chem B 124 9513-9525 (2020)
PMID: 33073990

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.26 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 51-237; Coverage: 79%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand QCA 2 x QCA

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 47.947Å b: 67.719Å c: 58.363Å
α: 90° β: 102.28° γ: 90°
R-values:
R R work R free
0.161 0.159 0.181
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

Photoactive Yellow Protein Represents a Distinct, Evolutionarily Novel Family of PAS Domains.
Xing et al. (2022)
4 more

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