6ut8

Electron Microscopy
3.68Å resolution

Refined half-complex from tetradecameric assembly of Thermococcus gammatolerans McrB AAA+ hexamers with bound McrC

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-111439 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AAA+ ATPase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 428 amino acids
Theoretical weight: 50.14 KDa
Source organism: Thermococcus gammatolerans
Expression system: Escherichia coli
UniProt:
  • Canonical: C5A3Z3 (Residues: 186-613; Coverage: 70%)
Gene name: TGAM_0453
Sequence domains: AAA domain (dynein-related subfamily)
McrBC 5-methylcytosine restriction system component Chain: G
Molecule details ›
Chain: G
Length: 458 amino acids
Theoretical weight: 54.34 KDa
Source organism: Thermococcus gammatolerans
Expression system: Escherichia coli
UniProt:
  • Canonical: C5A3Z2 (Residues: 1-458; Coverage: 100%)
Gene name: TGAM_0452
Sequence domains: McrBC 5-methylcytosine restriction system component

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.68Å
Relevant EMDB volumes: EMD-20871
Expression system: Escherichia coli