› 6uu1

X-ray diffraction
4.1Å resolution

E. coli sigma-S transcription initiation complex with a 4-nt RNA and a CTP ("Fresh" crystal soaked with CTP, GTP, and ddTTP for 30 minutes)

Released:
DOI: 10.2210/pdb6uu1/pdb
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PDB entry 6uu1 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insights into Transcription Initiation from De Novo RNA Synthesis to Transitioning into Elongation.
Zuo Y, De S, Feng Y, Steitz TA
OpenAccess logo iScience 23 101445 (2020)
PMID: 32829286

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238475 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein; DNA-directed RNA polymerase subunit alpha Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 242 amino acids
Theoretical weight: 26.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-235; Coverage: 71%)
  • Canonical: A0A377D9Q8 (Residues: 1-235; Coverage: 91%)
Gene names: JW3257, NCTC9962_06804, b3295, pez, phs, rpoA, rpoA_1, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: CCC
Molecule details ›
Chain: CCC
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: DDD
DNA-directed RNA polymerase subunit omega Chain: EEE
RNA polymerase sigma factor RpoS Chain: FFF
Synthetic DNA 50-MER (promoter non-template strand) Chain: 111
Synthetic DNA 50-MER (promoter template strand) Chain: 222
RNA 4-mer Chain: 333

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand CTP 1 x CTP
2 modified residues:
Ligand GTP 1 x GTP
Ligand 2DT 1 x 2DT

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 132.854Å b: 154.252Å c: 230.729Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.335 0.332 0.396
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The Context-Dependent Influence of Promoter Sequence Motifs on Transcription Initiation Kinetics and Regulation.
Jensen et al. (2021)
2 other articles

1 mention without citation

Structural Insights into Transcription Initiation from De Novo RNA Synthesis to Transitioning into Elongation.
Zuo et al. (2020)

PDB-REDO

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