› 6ux8

X-ray diffraction
2.5Å resolution

Structure of monobody 33 MLKL N-terminal domain complex

Released:
DOI: 10.2210/pdb6ux8/pdb
Model geometry
Data not analysed
Fit model/data
Data not analysed
PDB entry 6ux8 coloured by chain, front view.
PDB entry 6ux8 coloured by chain, side view.
PDB entry 6ux8 coloured by chain, top view.
Source organisms:
Primary publication:
Identification of MLKL membrane translocation as a checkpoint in necroptotic cell death using Monobodies.
Petrie EJ, Birkinshaw RW, Koide A, Denbaum E, Hildebrand JM, Garnish SE, Davies KA, Sandow JJ, Samson AL, Gavin X, Fitzgibbon C, Young SN, Hennessy PJ, Smith PPC, Webb AI, Czabotar PE, Koide S, Murphy JM
Proc Natl Acad Sci U S A 117 8468-8475 (2020)
PMID: 32234780

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167585 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mixed lineage kinase domain-like protein Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 18.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NB16 (Residues: 5-154; Coverage: 32%)
Gene name: MLKL
Sequence domains: Mixed lineage kinase domain-like N-terminal domain
Monobody Chain: B
Molecule details ›
Chain: B
Length: 97 amino acids
Theoretical weight: 10.43 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 58.294Å b: 59.418Å c: 68.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.247 0.296
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Necroptosis in Immuno-Oncology and Cancer Immunotherapy.
Sprooten et al. (2020)
9 more

4 mentions without citation

Monobodies as tool biologics for accelerating target validation and druggable site discovery.
Akkapeddi et al. (2021)
3 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO