› 6uxe

X-ray diffraction
1.57Å resolution

Structure of the human mitochondrial desulfurase complex Nfs1-ISCU2(M140I)-ISD11 with E.coli ACP1 at 1.57 A resolution showing flexibility of N terminal end of ISCU2

Released:
DOI: 10.2210/pdb6uxe/pdb
PDB entry 6uxe coloured by chain, front view.
PDB entry 6uxe coloured by chain, side view.
PDB entry 6uxe coloured by chain, top view.
Source organisms:
Entry authors: Boniecki MT, Cygler M

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-141284 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cysteine desulfurase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 45.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y697 (Residues: 56-457; Coverage: 88%)
Gene names: HUSSY-08, NFS1, NIFS
Sequence domains: Aminotransferase class-V
LYR motif-containing protein 4 Chain: B
Molecule details ›
Chain: B
Length: 91 amino acids
Theoretical weight: 10.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HD34 (Residues: 1-91; Coverage: 100%)
Gene names: C6orf149, CGI-203, ISD11, LYRM4
Sequence domains: Complex 1 protein (LYR family)
Acyl carrier protein Chain: C
Molecule details ›
Chain: C
Length: 77 amino acids
Theoretical weight: 8.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6A8 (Residues: 2-78; Coverage: 99%)
Gene names: JW1080, acpP, b1094
Sequence domains: Phosphopantetheine attachment site
Iron-sulfur cluster assembly enzyme ISCU Chain: D
Molecule details ›
Chain: D
Length: 143 amino acids
Theoretical weight: 15.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H1K1 (Residues: 35-167; Coverage: 80%)
Gene names: ISCU, NIFUN
Sequence domains: NifU-like N terminal domain

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
11 bound ligands:
Ligand GOL 6 x GOL
Ligand EDO 50 x EDO
Ligand PEG 11 x PEG
Ligand PG4 2 x PG4
Ligand P15 1 x P15
Ligand DTT 1 x DTT
Ligand PGE 3 x PGE
Ligand EDT 1 x EDT
Ligand 8Q1 1 x 8Q1
Ligand MES 1 x MES
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41212
Unit cell:
a: 86.41Å b: 86.41Å c: 245.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.18
Expression system: Escherichia coli BL21(DE3)

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