› 6uzx

X-ray diffraction
2.41Å resolution

Crystal structure of GLUN1/GLUN2A-4M mutant ligand-binding domain in complex with glycine and UBP791

Released:
DOI: 10.2210/pdb6uzx/pdb
PDB entry 6uzx coloured by chain, front view.
PDB entry 6uzx coloured by chain, side view.
PDB entry 6uzx coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural basis of subtype-selective competitive antagonism for GluN2C/2D-containing NMDA receptors.
Wang JX, Irvine MW, Burnell ES, Sapkota K, Thatcher RJ, Li M, Simorowski N, Volianskis A, Collingridge GL, Monaghan DT, Jane DE, Furukawa H
OpenAccess logo Nat Commun 11 423 (2020)
PMID: 31969570

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152907 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutamate receptor ionotropic, NMDA 1 Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.34 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35439 (Residues: 394-544, 663-800; Coverage: 31%)
Gene names: Grin1, Nmdar1
Sequence domains:
Glutamate receptor ionotropic, NMDA 2A Chain: B
Molecule details ›
Chain: B
Length: 282 amino acids
Theoretical weight: 31.86 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00959 (Residues: 402-539, 661-802; Coverage: 19%)
Gene name: Grin2a
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand GLY 1 x GLY
Ligand QM4 1 x QM4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P212121
Unit cell:
a: 58.987Å b: 85.081Å c: 120.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.
Hansen et al. (2021)
3 more