› 6v1r

X-ray diffraction
1.64Å resolution

Crystal structure of iAChSnFR Fluorescent Acetylcholine Sensor precursor binding protein

Released:
DOI: 10.2210/pdb6v1r/pdb
PDB entry 6v1r coloured by chain, front view.
PDB entry 6v1r coloured by chain, side view.
PDB entry 6v1r coloured by chain, top view.
Source organism: Thermoanaerobacter sp. X513
Entry authors: Fan C, Borden PM, Looger LL, Lester HA, Rees DC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC-type glycine betaine transport system substrate-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33 KDa
Source organism: Thermoanaerobacter sp. X513
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3B8MYE1 (Residues: 28-304; Coverage: 91%)
Gene name: DCE09_00900
Sequence domains: Substrate binding domain of ABC-type glycine betaine transport system

Ligands and Environments

4 bound ligands:
Ligand ACH 1 x ACH
Ligand NHE 1 x NHE
Ligand SPD 1 x SPD
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 46.76Å b: 65.781Å c: 96.592Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.221
Expression system: Escherichia coli BL21(DE3)

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