› 6v3y

X-ray diffraction
1.63Å resolution

Crystal structure of EED in complex with PALI1-K1219me3 peptide

Released:
DOI: 10.2210/pdb6v3y/pdb
PDB entry 6v3y coloured by chain, front view.
PDB entry 6v3y coloured by chain, side view.
PDB entry 6v3y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
PALI1 facilitates DNA and nucleosome binding by PRC2 and triggers an allosteric activation of catalysis.
Zhang Q, Agius SC, Flanigan SF, Uckelmann M, Levina V, Owen BM, Davidovich C
Nat Commun 12 4592 (2021)
PMID: 34321472

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131032 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb protein EED Chain: A
Molecule details ›
Chain: A
Length: 359 amino acids
Theoretical weight: 41.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75530 (Residues: 81-439; Coverage: 81%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Ligand-dependent corepressor Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 665 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96JN0 (Residues: 5-9; Coverage: 1%)
Gene names: C10orf12, KIAA1795, LCOR, MLR2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 57.775Å b: 85.296Å c: 91.106Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.192
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Evolutionary adaptation of the Polycomb repressive complex 2.
Fischer et al. (2022)
5 more

1 mention without citation

PALI1 facilitates DNA and nucleosome binding by PRC2 and triggers an allosteric activation of catalysis.
Zhang et al. (2021)