› 6vbw

Electron Microscopy
3.2Å resolution

Cryo-EM structure of Cascade-TniQ-dsDNA ternary complex

Released:
DOI: 10.2210/pdb6vbw/pdb
PDB entry 6vbw coloured by chain, front view.
PDB entry 6vbw coloured by chain, side view.
PDB entry 6vbw coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structure-function insights into the initial step of DNA integration by a CRISPR-Cas-Transposon complex.
Jia N, Xie W, de la Cruz MJ, Eng ET, Patel DJ
OpenAccess logo Cell Res 30 182-184 (2020)
PMID: 31925391
Related structures: EMD-21146

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-120752 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (7 distinct):
Cas8 Chain: A
Molecule details ›
Chain: A
Length: 640 amino acids
Theoretical weight: 72.29 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
Cas7 Chains: B, C, D, E, F, G
Molecule details ›
Chains: B, C, D, E, F, G
Length: 352 amino acids
Theoretical weight: 39.89 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
Cas6 Chain: H
TniQ Chains: I, J
DNA (5'-D(*GP*CP*AP*GP*TP*CP*AP*TP*CP*AP*CP*CP*AP*AP*TP*TP*TP*AP*TP*TP*TP*A)-3') Chain: M
DNA (100-MER) Chain: L
RNA (61-MER) Chain: K

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-21146
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structural biology of CRISPR-Cas immunity and genome editing enzymes.
Wang et al. (2022)
3 more

2 mentions without citation

Evolutionary and mechanistic diversity of Type I-F CRISPR-associated transposons.
Klompe et al. (2022)
1 more