› 6vcw

X-ray diffraction
1.4Å resolution

Crystal structure of Medicago truncatula S-adenosylmethionine Synthase 3A (MtMAT3A)

Released:
DOI: 10.2210/pdb6vcw/pdb
PDB entry 6vcw coloured by chain, front view.
PDB entry 6vcw coloured by chain, side view.
PDB entry 6vcw coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
S-adenosylmethionine synthases in plants: Structural characterization of type I and II isoenzymes from Arabidopsis thaliana and Medicago truncatula.
Sekula B, Ruszkowski M, Dauter Z
Int J Biol Macromol 151 554-565 (2020)
PMID: 32057875

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 42.98 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G7L3W1 (Residues: 1-390; Coverage: 100%)
Gene names: 11432371, MTR_7g102120, MtrunA17_Chr7g0264651
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand CL 2 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 111.602Å b: 62.042Å c: 102.284Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.115 0.115 0.159
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The Role of the m6A RNA Methyltransferase METTL16 in Gene Expression and SAM Homeostasis.
Mermoud JE. (2022)
4 other articles

2 mentions without citation

C-H Groups as Donors in Hydrogen Bonds: A Historical Overview and Occurrence in Proteins and Nucleic Acids.
Derewenda ZS. (2023)
1 more