› 6vfo

Solution NMR

Solution structure of the PHD of mouse UHRF1 (NP95)

Released:
DOI: 10.2210/pdb6vfo/pdb
PDB entry 6vfo coloured by chain, ensemble of 20 models, front view.
PDB entry 6vfo coloured by chain, ensemble of 20 models, side view.
PDB entry 6vfo coloured by chain, ensemble of 20 models, top view.
Source organism: Mus musculus
Primary publication:
Alternative splicing and allosteric regulation modulate the chromatin binding of UHRF1.
Tauber M, Kreuz S, Lemak A, Mandal P, Yerkesh Z, Veluchamy A, Al-Gashgari B, Aljahani A, Cortés-Medina LV, Azhibek D, Fan L, Ong MS, Duan S, Houliston S, Arrowsmith CH, Fischle W
OpenAccess logo Nucleic Acids Res 48 7728-7747 (2020)
PMID: 32609811

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186561 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase UHRF1 Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 8.69 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VDF2 (Residues: 303-380; Coverage: 10%)
Gene names: Np95, Uhrf1
Sequence domains: PHD-finger

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The interplay between DNA and histone methylation: molecular mechanisms and disease implications.
Li et al. (2021)
4 more

2 mentions without citation

Alternative splicing and allosteric regulation modulate the chromatin binding of UHRF1.
Tauber et al. (2020)
1 more