› 6vjk

X-ray diffraction
1.6Å resolution

Streptavidin mutant M88 (N49C/A86C)

Released:
DOI: 10.2210/pdb6vjk/pdb
PDB entry 6vjk coloured by chain, front view.
PDB entry 6vjk coloured by chain, side view.
PDB entry 6vjk coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity.
Marangoni JM, Wu SC, Fogen D, Wong SL, Ng KKS
OpenAccess logo Sci Rep 10 12483 (2020)
PMID: 32719366

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 124 amino acids
Theoretical weight: 13.13 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 38-160; Coverage: 77%)
Sequence domains: Avidin family

Ligands and Environments

1 bound ligand:
Ligand BTN 12 x BTN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 60.26Å b: 79.549Å c: 281.549Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Binding Affinity Determination in Drug Design: Insights from Lock and Key, Induced Fit, Conformational Selection, and Inhibitor Trapping Models.
Spassov DS. (2024)

1 mention without citation

Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity.
Marangoni et al. (2020)