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CryoEM structure of Hrd3/Yos9 complex

Electron Microscopy
3.7Å resolution
PDB entry 6vk3 coloured by chain, front view.
PDB entry 6vk3 coloured by chain, side view.
PDB entry 6vk3 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: ERAD-associated E3 ubiquitin-protein ligase component HRD3 and Protein OS-9 homolog
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Multimeric state: hetero dimer
Accessible surface area: 46833.08 Å2
Buried surface area: 5001.4 Å2
Dissociation area: 2,500.7 Å2
Dissociation energy (ΔGdiss): 19.11 kcal/mol
Dissociation entropy (TΔSdiss): 14.9 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-170250

Macromolecules

ERAD-associated E3 ubiquitin-protein ligase component HRD3
Chain: A
Length: 732 amino acids
Theoretical weight: 84.33 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q05787 (Residues: 1-767; Coverage: 88%)
Gene names: HRD3, YLR207W
Pfam: Sel1 repeat
InterPro:
ERAD-associated E3 ubiquitin-protein ligase component HRD3 in PDB entry 6vk3, assembly 1, front view.
ERAD-associated E3 ubiquitin-protein ligase component HRD3 in PDB entry 6vk3, assembly 1, side view.
ERAD-associated E3 ubiquitin-protein ligase component HRD3 in PDB entry 6vk3, assembly 1, top view.
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Protein OS-9 homolog
Chain: B
Length: 542 amino acids
Theoretical weight: 61.31 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q99220 (Residues: 1-542; Coverage: 100%)
Gene names: D4222, YDR057W, YOS9
Pfam:
InterPro:
Protein OS-9 homolog in PDB entry 6vk3, assembly 1, front view.
Protein OS-9 homolog in PDB entry 6vk3, assembly 1, side view.
Protein OS-9 homolog in PDB entry 6vk3, assembly 1, top view.
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