› 6vk5

X-ray diffraction
1.86Å resolution

Crystal Structure of Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase and Regulatory Component Complex

Released:
DOI: 10.2210/pdb6vk5/pdb
PDB entry 6vk5 coloured by chain, front view.
PDB entry 6vk5 coloured by chain, side view.
PDB entry 6vk5 coloured by chain, top view.
Source organism: Methylosinus trichosporium OB3b
Primary publication:
Structural Studies of the Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase and Regulatory Component Complex Reveal a Transient Substrate Tunnel.
Jones JC, Banerjee R, Shi K, Aihara H, Lipscomb JD
Biochemistry 59 2946-2961 (2020)
PMID: 32692178

Function and Biology Details

Reaction catalysed: 
Methane + NAD(P)H + O(2) = methanol + NAD(P)(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-104203 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Methane monooxygenase component A alpha chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 526 amino acids
Theoretical weight: 60.03 KDa
Source organism: Methylosinus trichosporium OB3b
UniProt:
  • Canonical: P27353 (Residues: 1-526; Coverage: 100%)
Gene name: mmoX
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Methane monooxygenase Chains: B, F
Molecule details ›
Chains: B, F
Length: 395 amino acids
Theoretical weight: 45.3 KDa
Source organism: Methylosinus trichosporium OB3b
UniProt:
  • Canonical: A0A2D2D5X7 (Residues: 1-395; Coverage: 100%)
Gene name: CQW49_12475
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Methane monooxygenase Chains: C, G
Molecule details ›
Chains: C, G
Length: 169 amino acids
Theoretical weight: 19.44 KDa
Source organism: Methylosinus trichosporium OB3b
UniProt:
  • Canonical: A0A2D2D0T0 (Residues: 1-169; Coverage: 100%)
Gene name: CQW49_12465
Sequence domains: Methane monooxygenase, hydrolase gamma chain
Methane monooxygenase regulatory protein B Chains: D, H
Molecule details ›
Chains: D, H
Length: 138 amino acids
Theoretical weight: 14.9 KDa
Source organism: Methylosinus trichosporium OB3b
UniProt:
  • Canonical: P27356 (Residues: 1-138; Coverage: 100%)
Gene name: mmoB
Sequence domains: MmoB/DmpM family

Ligands and Environments

4 bound ligands:
Ligand FE 4 x FE
Ligand EDO 30 x EDO
Ligand BEZ 2 x BEZ
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 102.615Å b: 105.459Å c: 299.421Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.193

Quick links

Citations

4 review citations

Biochemistry of aerobic biological methane oxidation.
Koo et al. (2021)
3 more

4 mentions without citation

Structural Studies of the Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase and Regulatory Component Complex Reveal a Transient Substrate Tunnel.
Jones et al. (2020)
3 more