› 6vkl

Electron Microscopy
15Å resolution

Negative stain reconstruction of the yeast exocyst octameric complex.

Released:
DOI: 10.2210/pdb6vkl/pdb
PDB entry 6vkl coloured by chain, front view.
PDB entry 6vkl coloured by chain, side view.
PDB entry 6vkl coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Exocyst structural changes associated with activation of tethering downstream of Rho/Cdc42 GTPases.
Rossi G, Lepore D, Kenner L, Czuchra AB, Plooster M, Frost A, Munson M, Brennwald P
OpenAccess logo J Cell Biol 219 (2020)
PMID: 31904797
Related structures: EMD-21226

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148681 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Exocyst complex component SEC3 Chain: A
Molecule details ›
Chain: A
Length: 1336 amino acids
Theoretical weight: 154.89 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33332 (Residues: 1-1336; Coverage: 100%)
Gene names: PSL1, SEC3, YER008C
Sequence domains:
Exocyst complex component SEC5 Chain: B
Molecule details ›
Chain: B
Length: 971 amino acids
Theoretical weight: 112.24 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P89102 (Residues: 1-971; Coverage: 100%)
Gene names: SEC5, YD8358.20C, YD9489.01C, YDR166C
Sequence domains: Exocyst complex component Sec5
Exocyst complex component SEC6 Chain: C
Molecule details ›
Chain: C
Length: 805 amino acids
Theoretical weight: 93.54 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32844 (Residues: 1-805; Coverage: 100%)
Gene names: SEC6, YIL068C
Sequence domains: Exocyst complex component Sec6
Exocyst complex component SEC8 Chain: D
Molecule details ›
Chain: D
Length: 1065 amino acids
Theoretical weight: 122.37 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32855 (Residues: 1-1065; Coverage: 100%)
Gene names: SEC8, YP9499.11, YPR055W
Sequence domains:
Exocyst complex component SEC10 Chain: E
Molecule details ›
Chain: E
Length: 871 amino acids
Theoretical weight: 100.46 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06245 (Residues: 1-871; Coverage: 100%)
Gene names: L9362.12, SEC10, YLR166C
Sequence domains:
Exocyst complex component SEC15 Chain: F
Molecule details ›
Chain: F
Length: 910 amino acids
Theoretical weight: 105.17 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P22224 (Residues: 1-910; Coverage: 100%)
Gene names: SEC15, YGL233W
Sequence domains:
Exocyst complex component EXO70 Chain: G
Molecule details ›
Chain: G
Length: 623 amino acids
Theoretical weight: 71.38 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P19658 (Residues: 1-623; Coverage: 100%)
Gene names: EXO70, J0932, YJL085W
Sequence domains:
Exocyst complex component EXO84 Chain: H
Molecule details ›
Chain: H
Length: 753 amino acids
Theoretical weight: 85.65 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38261 (Residues: 1-753; Coverage: 100%)
Gene names: EXO84, USA3, YBR0831, YBR102C
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 15Å
Relevant EMDB volumes: EMD-21226

Quick links

Citations

3 review citations

Golgi inCOGnito: From vesicle tethering to human disease.
D'Souza et al. (2020)
2 more

1 mention without citation

Allosteric regulation of exocyst: Discrete activation of tethering by two spatial signals.
Miller et al. (2023)