› 6vp3

X-ray diffraction
1.65Å resolution

Artificial Metalloproteins with Dinuclear Iron Centers

Released:
DOI: 10.2210/pdb6vp3/pdb
PDB entry 6vp3 coloured by chain, front view.
PDB entry 6vp3 coloured by chain, side view.
PDB entry 6vp3 coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Artificial Metalloproteins with Dinuclear Iron-Hydroxido Centers.
Miller KR, Biswas S, Jasniewski A, Follmer AH, Biswas A, Albert T, Sabuncu S, Bominaar EL, Hendrich MP, Moënne-Loccoz P, Borovik AS
J Am Chem Soc 143 2384-2393 (2021)
PMID: 33528256

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 159 amino acids
Theoretical weight: 16.56 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 38-183; Coverage: 92%)
Sequence domains: Avidin family

Ligands and Environments

2 bound ligands:
Ligand KM3 4 x KM3
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 193.503Å b: 57.861Å c: 57.861Å
α: 90° β: 107.4° γ: 90°
R-values:
R R work R free
0.178 0.176 0.221
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Artificial Metalloproteins: At the Interface between Biology and Chemistry.
Kerns et al. (2022)
1 more