› 6vqw

Electron Microscopy
3.42Å resolution

Type I-F CRISPR-Csy complex with its inhibitor AcrF8

Released:
DOI: 10.2210/pdb6vqw/pdb
PDB entry 6vqw coloured by chain, front view.
PDB entry 6vqw coloured by chain, side view.
PDB entry 6vqw coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Inhibition mechanisms of AcrF9, AcrF8, and AcrF6 against type I-F CRISPR-Cas complex revealed by cryo-EM.
Zhang K, Wang S, Li S, Zhu Y, Pintilie GD, Mou TC, Schmid MF, Huang Z, Chiu W
Proc Natl Acad Sci U S A 117 7176-7182 (2020)
PMID: 32170016
Related structures: EMD-21359

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-167655 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (6 distinct):
CRISPR-associated protein Csy2 Chain: C
Molecule details ›
Chain: C
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: D, E, F, G, H, I
Molecule details ›
Chains: D, E, F, G, H, I
Length: 360 amino acids
Theoretical weight: 39.78 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q02MM1 (Residues: 2-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
CRISPR-associated endonuclease Cas6/Csy4 Chain: J
Molecule details ›
Chain: J
Length: 187 amino acids
Theoretical weight: 21.43 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
AcrF8 Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.21 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
CRISPR-associated protein Csy1 Chain: B
Molecule details ›
Chain: B
Length: 434 amino acids
Theoretical weight: 49.19 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CrRNA (40-MER) Chain: K
Molecule details ›
Chain: K
Length: 60 nucleotides
Theoretical weight: 19.25 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.42Å
Relevant EMDB volumes: EMD-21359
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

3 review citations

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.
Jia et al. (2021)
2 more

2 mentions without citation

Structural insights into the inactivation of the type I-F CRISPR-Cas system by anti-CRISPR proteins.
Yang et al. (2021)
1 more