› 6vrp

X-ray diffraction
1.6Å resolution

Reverse Transcriptase Diabody with R83T Mutation

Released:
DOI: 10.2210/pdb6vrp/pdb
PDB entry 6vrp coloured by chain, front view.
PDB entry 6vrp coloured by chain, side view.
PDB entry 6vrp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Co-crystallization with diabodies: A case study for the introduction of synthetic symmetry.
Chesterman C, Arnold E
Structure 29 598-605.e3 (2021)
PMID: 33636101

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-chain Fv Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 26.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand EDO 13 x EDO
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 52.728Å b: 55.46Å c: 157.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.183
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Modeling and Structure Determination of Homo-Oligomeric Proteins: An Overview of Challenges and Current Approaches.
Gaber et al. (2021)
1 more

1 mention without citation

Co-crystallization with diabodies: A case study for the introduction of synthetic symmetry.
Chesterman et al. (2021)