› 6vsl

X-ray diffraction
2.1Å resolution

Crystal structure of a human fucosylated IgG1 Fc expressed in tobacco plants (Nicotiana benthamiana)

Released:
DOI: 10.2210/pdb6vsl/pdb
PDB entry 6vsl coloured by chain, front view.
PDB entry 6vsl coloured by chain, side view.
PDB entry 6vsl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Enhanced Ability of Plant-Derived PGT121 Glycovariants To Eliminate HIV-1-Infected Cells.
Anand SP, Ding S, Tolbert WD, Prévost J, Richard J, Gil HM, Gendron-Lepage G, Cheung WF, Wang H, Pastora R, Saxena H, Wakarchuk W, Medjahed H, Wines BD, Hogarth M, Shaw GM, Martin MA, Burton DR, Hangartner L, Evans DT, Pazgier M, Cossar D, McLean MD, Finzi A
J Virol 95 e0079621 (2021)
PMID: 34232070

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Immunoglobulin gamma-1 heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.92 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: P0DOX5 (Residues: 236-446; Coverage: 47%)
Sequence domains: Immunoglobulin C1-set domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUC
Carbohydrate polymer
1 bound ligand:
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P212121
Unit cell:
a: 49.86Å b: 79.92Å c: 138.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.25
Expression system: Nicotiana benthamiana

Quick links

Citations

1 review citation

Natural Killer Cells in Antibody Independent and Antibody Dependent HIV Control.
Bernard et al. (2022)
3 other articles

1 mention without citation

Enhanced Ability of Plant-Derived PGT121 Glycovariants To Eliminate HIV-1-Infected Cells.
Anand et al. (2021)