› 6vwo

X-ray diffraction
1.78Å resolution

Crystal structure of E. coli guanosine kinase

Released:
DOI: 10.2210/pdb6vwo/pdb
PDB entry 6vwo coloured by chain, front view.
PDB entry 6vwo coloured by chain, side view.
PDB entry 6vwo coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
ppGpp Coordinates Nucleotide and Amino-Acid Synthesis in E. coli During Starvation.
Wang B, Grant RA, Laub MT
Mol Cell 80 29-42.e10 (2020)
PMID: 32857952

Function and Biology Details

Reaction catalysed: 
ATP + inosine = ADP + IMP
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanosine-inosine kinase Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 48.78 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEW6 (Residues: 1-434; Coverage: 100%)
Gene names: JW0466, b0477, gsk
Sequence domains: pfkB family carbohydrate kinase

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand GMP 2 x GMP
Ligand K 3 x K
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3221
Unit cell:
a: 118.117Å b: 118.117Å c: 75.447Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.181 0.205
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Possible Roles for Basal Levels of (p)ppGpp: Growth Efficiency Vs. Surviving Stress.
Fernández-Coll et al. (2020)
7 more

1 mention without citation

ppGpp Coordinates Nucleotide and Amino-Acid Synthesis in E. coli During Starvation.
Wang et al. (2020)