› 6vz8

Electron Microscopy
3.45Å resolution

Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound

Released:
DOI: 10.2210/pdb6vz8/pdb
PDB entry 6vz8 coloured by chain, front view.
PDB entry 6vz8 coloured by chain, side view.
PDB entry 6vz8 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structures of fungal and plant acetohydroxyacid synthases.
Lonhienne T, Low YS, Garcia MD, Croll T, Gao Y, Wang Q, Brillault L, Williams CM, Fraser JA, McGeary RP, West NP, Landsberg MJ, Rao Z, Schenk G, Guddat LW
Nature 586 317-321 (2020)
PMID: 32640464
Related structures: EMD-21487

Function and Biology Details

Reaction catalysed: 
2 pyruvate = 2-acetolactate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148107 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acetolactate synthase, chloroplastic Chains: D, E, H, I, L, M, P, Q
Molecule details ›
Chains: D, E, H, I, L, M, P, Q
Length: 586 amino acids
Theoretical weight: 64.03 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P17597 (Residues: 86-670; Coverage: 87%)
Gene names: AHAS, ALS, At3g48560, CSR1, T8P19.70, TZP5
Sequence domains:
Acetolactate synthase small subunit 1, chloroplastic Chains: F, G, J, K, N, O, R, S
Molecule details ›
Chains: F, G, J, K, N, O, R, S
Length: 491 amino acids
Theoretical weight: 53.95 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93YZ7 (Residues: 1-491; Coverage: 100%)
Gene names: AHASS1, AIP1, At2g31810, F20M17.15
Sequence domains:

Ligands and Environments


Cofactor: Ligand TPP 8 x TPP

Cofactor: Ligand FAD 8 x FAD
2 bound ligands:
Ligand MG 8 x MG
Ligand VAL 8 x VAL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.45Å
Relevant EMDB volumes: EMD-21487
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.
Priest et al. (2021)
4 more

1 mention without citation

Genome-Guided Analysis of Seven Weed Species Reveals Conserved Sequence and Structural Features of Key Gene Targets for Herbicide Development.
Shah et al. (2022)