6w20

Electron Microscopy
3Å resolution

ClpAP Disengaged State bound to RepA-GFP

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
PDBe Complex ID:
PDB-CPX-141332 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP-dependent Clp protease proteolytic subunit Chains: G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 207 amino acids
Theoretical weight: 23.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
ATP-dependent Clp protease ATP-binding subunit ClpA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 758 amino acids
Theoretical weight: 84.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
RepA, green fluorescent protein fusion Chain: X

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-21520
Expression system: Escherichia coli