6w2t

Electron Microscopy
3.36Å resolution

Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2)

Released:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 44-mer (preferred)
PDBe Complex ID:
PDB-CPX-244009 (preferred)
Entry contents:
42 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (44 distinct):
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 295 amino acids
Theoretical weight: 33.02 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein eS1 Chain: C
Molecule details ›
Chain: C
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein uS5 Chain: D
Small ribosomal subunit protein eS4 Chain: F
Small ribosomal subunit protein eS6 Chain: H
Small ribosomal subunit protein eS7 Chain: I
Small ribosomal subunit protein eS8 Chain: J
Small ribosomal subunit protein uS4 Chain: K
Small ribosomal subunit protein uS17 Chain: M
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein uS11 Chain: P
eS21 Chain: W
Small ribosomal subunit protein uS8 Chain: X
Small ribosomal subunit protein uS12 Chain: Y
40S ribosomal protein S24 Chain: Z
Small ribosomal subunit protein eS26 Chain: b
Small ribosomal subunit protein eS27 Chain: c
Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein Chain: f
Small ribosomal subunit protein uS3 Chain: E
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein eS10 Chain: L
Small ribosomal subunit protein eS12 Chain: N
Small ribosomal subunit protein uS19 Chain: Q
Small ribosomal subunit protein uS9 Chain: R
Small ribosomal subunit protein eS17 Chain: S
Small ribosomal subunit protein uS13 Chain: T
Small ribosomal subunit protein eS19 Chain: U
Small ribosomal subunit protein uS10 Chain: V
Small ribosomal subunit protein eS25 Chain: i
Small ribosomal subunit protein eS28 Chain: d
Small ribosomal subunit protein uS14 Chain: e
Ubiquitin-ribosomal protein eS31 fusion protein Chain: g
Small ribosomal subunit protein RACK1 Chain: h
Eukaryotic translation initiation factor 3 subunit E Chain: 3
Eukaryotic translation initiation factor 3 subunit F Chain: 4
Eukaryotic translation initiation factor 3 subunit K Chain: 6
Eukaryotic translation initiation factor 3 subunit L Chain: 7
Eukaryotic translation initiation factor 3 subunit M Chain: 8
Eukaryotic translation initiation factor 3 subunit A Chain: 1
Eukaryotic translation initiation factor 3 subunit C Chain: 2
Eukaryotic translation initiation factor 3 subunit D Chain: 9
Eukaryotic translation initiation factor 3 subunit H Chain: 5
18S Ribosomal RNA Chain: a
CrPV 5'-UTR IRES Chain: A

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.36Å
Relevant EMDB volumes: EMD-21530
Expression system: Escherichia coli