6w5n

Electron Microscopy
6Å resolution

Cryo-EM structure of MLL1 in complex with RbBP5, WDR5, SET1, and ASH2L bound to the nucleosome (Class05)

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-120809 (preferred)
Entry contents:
8 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (10 distinct):
Retinoblastoma-binding protein 5 Chain: A
Molecule details ›
Chain: A
Length: 538 amino acids
Theoretical weight: 59.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15291 (Residues: 2-538; Coverage: 100%)
Gene names: RBBP5, RBQ3
Sequence domains: WD domain, G-beta repeat
WD repeat-containing protein 5 Chain: B
Molecule details ›
Chain: B
Length: 313 amino acids
Theoretical weight: 34.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 22-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
MLL cleavage product C180 Chain: C
Set1/Ash2 histone methyltransferase complex subunit ASH2 Chain: D
Histone H3.2 Chains: G, K
Histone H4 Chains: H, L
Histone H2A type 1 Chains: I, M
Histone H2B 1.1 Chains: J, N
DNA (147-MER) Chain: O
DNA (147-MER) Chain: P

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-21544
Expression system: Escherichia coli