› 6w66

X-ray diffraction
3.21Å resolution

The structure of the F64A, S172A mutant Keap1-BTB domain in complex with SKP1-FBXL17

Released:
DOI: 10.2210/pdb6w66/pdb
PDB entry 6w66 coloured by chain, front view.
PDB entry 6w66 coloured by chain, side view.
PDB entry 6w66 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for dimerization quality control.
Mena EL, Jevtić P, Greber BJ, Gee CL, Lew BG, Akopian D, Nogales E, Kuriyan J, Rape M
OpenAccess logo Nature 586 452-456 (2020)
PMID: 32814905

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-159039 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63208 (Residues: 1-163; Coverage: 100%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
F-box/LRR-repeat protein 17 Chain: B
Molecule details ›
Chain: B
Length: 396 amino acids
Theoretical weight: 44.97 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9UF56 (Residues: 310-701; Coverage: 56%)
Gene names: FBL17, FBX13, FBXL17, FBXO13
Sequence domains:
Kelch-like ECH-associated protein 1 Chain: C
Molecule details ›
Chain: C
Length: 134 amino acids
Theoretical weight: 14.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14145 (Residues: 48-180; Coverage: 21%)
Gene names: INRF2, KEAP1, KIAA0132, KLHL19
Sequence domains: BTB/POZ domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3221
Unit cell:
a: 183.665Å b: 183.665Å c: 55.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.252 0.29
Expression systems:
  • Escherichia coli BL21(DE3)
  • Trichoplusia ni

Quick links

Citations

2 review citations

Cullin-RING Ubiquitin Ligase Regulatory Circuits: A Quarter Century Beyond the F-Box Hypothesis.
Harper et al. (2021)
1 more

2 mentions without citation

Structural basis for dimerization quality control.
Mena et al. (2020)
1 more