› 6wce

X-ray diffraction
1.75Å resolution

Structure of the periplasmic binding protein P5PA

Released:
DOI: 10.2210/pdb6wce/pdb
PDB entry 6wce coloured by chain, front view.
PDB entry 6wce coloured by chain, side view.
PDB entry 6wce coloured by chain, top view.
Source organism: Actinobacillus pleuropneumoniae
Primary publication:
Actinobacillus utilizes a binding protein-dependent ABC transporter to acquire the active form of vitamin B6.
Pan C, Zimmer A, Shah M, Huynh MS, Lai CC, Sit B, Hooda Y, Curran DM, Moraes TF
OpenAccess logo J Biol Chem 297 101046 (2021)
PMID: 34358566

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron(III) transport system substrate-binding protein Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 39.16 KDa
Source organism: Actinobacillus pleuropneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0BUC2 (Residues: 1-343; Coverage: 100%)
Gene names: APJL_0552, afuA2
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

1 bound ligand:
Ligand PLP 1 x PLP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 51.468Å b: 42.273Å c: 69.601Å
α: 90° β: 106.818° γ: 90°
R-values:
R R work R free
0.162 0.161 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Listeria monocytogenes genes supporting growth under standard laboratory cultivation conditions and during macrophage infection.
Fischer et al. (2022)
2 more

1 mention without citation

Actinobacillus utilizes a binding protein-dependent ABC transporter to acquire the active form of vitamin B6.
Pan et al. (2021)