› 6wgl

X-ray diffraction
2.82Å resolution

Dupilumab fab with Crystal Kappa design complexed with human IL-4 receptor

Released:
DOI: 10.2210/pdb6wgl/pdb
PDB entry 6wgl coloured by chain, front view.
PDB entry 6wgl coloured by chain, side view.
PDB entry 6wgl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rapid and robust antibody Fab fragment crystallization utilizing edge-to-edge beta-sheet packing.
Lieu R, Antonysamy S, Druzina Z, Ho C, Kang NR, Pustilnik A, Wang J, Atwell S
PLoS One 15 e0232311 (2020)
PMID: 32915778

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210874 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Dupilumab Fab heavy chain Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 25.23 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Dupilumab Fab light chain Chain: B
Molecule details ›
Chain: B
Length: 217 amino acids
Theoretical weight: 23.81 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Soluble interleukin-4 receptor subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 218 amino acids
Theoretical weight: 24.91 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P24394 (Residues: 26-232; Coverage: 26%)
Gene names: 582J2.1, IL4R, IL4RA
Sequence domains: Interleukin-4 receptor alpha chain, N-terminal

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P43212
Unit cell:
a: 73.49Å b: 73.49Å c: 412.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.304
Expression system: Cricetulus griseus

Quick links

Citations

1 review citation

Massively-multiplexed epitope mapping techniques for viral antigen discovery.
Hu et al. (2023)
6 other articles

5 mentions without citation

Rapid and robust antibody Fab fragment crystallization utilizing edge-to-edge beta-sheet packing.
Lieu et al. (2020)
4 more