› 6whi

Electron Microscopy
4.2Å resolution

Cryo-electron microscopy structure of the type I-F CRISPR RNA-guided surveillance complex bound to the anti-CRISPR AcrIF9

Released:
DOI: 10.2210/pdb6whi/pdb
PDB entry 6whi coloured by chain, front view.
PDB entry 6whi coloured by chain, side view.
PDB entry 6whi coloured by chain, top view.
Source organisms:
Primary publication:
AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex.
Hirschi M, Lu WT, Santiago-Frangos A, Wilkinson R, Golden SM, Davidson AR, Lander GC, Wiedenheft B
OpenAccess logo Nat Commun 11 2730 (2020)
PMID: 32483187
Related structures: EMD-21517

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-282696 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.19 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
CRISPR-associated protein Csy2 Chain: B
Molecule details ›
Chain: B
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
CRISPR-associated protein Csy3 Chains: C, D, E, F, G, H
CRISPR-associated endonuclease Cas6/Csy4 Chain: L
anti-CRISPR AcrIF9 Chains: I, J
non-target dsDNA Chains: N, Q
non-target dsDNA Chains: O, P
RNA (60-MER) Chain: M

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-21517
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.
Jia et al. (2021)
4 more

1 mention without citation

Structural insights into the inactivation of the type I-F CRISPR-Cas system by anti-CRISPR proteins.
Yang et al. (2021)