› 6wi2

X-ray diffraction
1.95Å resolution

Structure of human mitochondrial complex Nfs1-ISCU2-ISD11 with E.coli ACP1 at 1.95 A resolution (NIAU)2. N-terminal mutation of ISCU2 (L35) traps Nfs1 Cys loop in the active site of ISCU2 without metal present.

Released:
DOI: 10.2210/pdb6wi2/pdb
Model geometry
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PDB entry 6wi2 coloured by chain, front view.
PDB entry 6wi2 coloured by chain, side view.
PDB entry 6wi2 coloured by chain, top view.
Source organisms:
Entry authors: Boniecki MT, Cygler M

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-141278 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cysteine desulfurase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 45.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y697 (Residues: 56-457; Coverage: 88%)
Gene names: HUSSY-08, NFS1, NIFS
Sequence domains: Aminotransferase class-V
LYR motif-containing protein 4 Chain: B
Molecule details ›
Chain: B
Length: 91 amino acids
Theoretical weight: 10.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HD34 (Residues: 1-91; Coverage: 100%)
Gene names: C6orf149, CGI-203, ISD11, LYRM4
Sequence domains: Complex 1 protein (LYR family)
Acyl carrier protein Chain: C
Iron-sulfur cluster assembly enzyme ISCU Chain: D

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
10 bound ligands:
Ligand EDO 36 x EDO
Ligand GOL 3 x GOL
Ligand PEG 10 x PEG
Ligand PG4 2 x PG4
Ligand ETE 1 x ETE
Ligand PGE 1 x PGE
Ligand MES 1 x MES
Ligand EDT 1 x EDT
Ligand 8Q1 1 x 8Q1
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41212
Unit cell:
a: 86.43Å b: 86.43Å c: 246.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.185
Expression system: Escherichia coli

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