› 6wnt

Electron Microscopy
3.1Å resolution

50S ribosomal subunit without free 5S rRNA and perturbed PTC

Released:
DOI: 10.2210/pdb6wnt/pdb
PDB entry 6wnt coloured by chain, front view.
PDB entry 6wnt coloured by chain, side view.
PDB entry 6wnt coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly.
Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vázquez-Laslop N, Korostelev AA, Mankin AS
OpenAccess logo Nat Commun 11 2900 (2020)
PMID: 32518240
Related structures: EMD-21856

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 31-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135806 (preferred)
Entry contents:
30 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (31 distinct):
Large ribosomal subunit protein uL2 Chain: b
Molecule details ›
Chain: b
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 2-272; Coverage: 99%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: c
Molecule details ›
Chain: c
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: d
Large ribosomal subunit protein uL5 Chain: e
Large ribosomal subunit protein uL6 Chain: f
Large ribosomal subunit protein bL9 Chain: g
Large ribosomal subunit protein uL10 Chain: h
Large ribosomal subunit protein uL11 Chain: i
Large ribosomal subunit protein uL13 Chain: j
Large ribosomal subunit protein uL14 Chain: k
Large ribosomal subunit protein uL15 Chain: l
Large ribosomal subunit protein bL17 Chain: n
Large ribosomal subunit protein uL18 Chain: o
Large ribosomal subunit protein bL19 Chain: p
Large ribosomal subunit protein bL20 Chain: q
Large ribosomal subunit protein bL21 Chain: r
Large ribosomal subunit protein uL22 Chain: s
Large ribosomal subunit protein uL23 Chain: t
Large ribosomal subunit protein uL24 Chain: u
Large ribosomal subunit protein bL25 Chain: v
Large ribosomal subunit protein bL27 Chain: w
Large ribosomal subunit protein bL28 Chain: x
Large ribosomal subunit protein uL29 Chain: y
Large ribosomal subunit protein uL30 Chain: z
Large ribosomal subunit protein bL31 Chain: A
Large ribosomal subunit protein bL32 Chain: B
Large ribosomal subunit protein bL34 Chain: D
Large ribosomal subunit protein bL35 Chain: E
Large ribosomal subunit protein bL36A Chain: F
Large ribosomal subunit protein uL1 Chain: a
23s-5s joint ribosomal RNA Chain: 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-21856

Quick links

Citations

4 review citations

RNA Polymerases I and III in development and disease.
Watt et al. (2023)
3 more