› 6wqa

X-ray diffraction
2Å resolution

2.0A angstrom A2a adenosine receptor structure using XFEL data collected in helium atmosphere.

Released:
DOI: 10.2210/pdb6wqa/pdb
PDB entry 6wqa coloured by chain, front view.
PDB entry 6wqa coloured by chain, side view.
PDB entry 6wqa coloured by chain, top view.
Source organisms:
Primary publication:
Harnessing the power of an X-ray laser for serial crystallography of membrane proteins crystallized in lipidic cubic phase
Lee M-Y, Geiger J, Ishchenko A, Han GW, Barty A, White TA, Gati C, Batyuk A, Hunter MS, Aquila A, Boutet S, Weierstall U, Cherezov V, Liu W
IUCrJ 7 976-984 (2020)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine receptor A2a; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.97 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29274 (Residues: 2-208, 219-316; Coverage: 74%)
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
Gene names: ADORA2, ADORA2A, cybC
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand NA 1 x NA
Ligand ZMA 1 x ZMA
Ligand CLR 3 x CLR
Ligand OLA 15 x OLA
Ligand OLC 5 x OLC
Ligand GOL 1 x GOL
Ligand OLB 1 x OLB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: C2221
Unit cell:
a: 40.35Å b: 180.5Å c: 142.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.216
Expression system: Spodoptera frugiperda

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