› 6wqs

X-ray diffraction
2Å resolution

Xanthomonas citri Methionyl-tRNA synthetase in complex with REP8839

Released:
DOI: 10.2210/pdb6wqs/pdb
PDB entry 6wqs coloured by chain, front view.
PDB entry 6wqs coloured by chain, side view.
PDB entry 6wqs coloured by chain, top view.
Source organism: Xanthomonas citri pv. citri str. 306
Primary publication:
Molecular basis for diaryldiamine selectivity and competition with tRNA in a type 2 methionyl-tRNA synthetase from a Gram-negative bacterium.
Mercaldi GF, Andrade MO, Zanella JL, Cordeiro AT, Benedetti CE
OpenAccess logo J Biol Chem 296 100658
PMID: 33857480

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185781 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 584 amino acids
Theoretical weight: 64.1 KDa
Source organism: Xanthomonas citri pv. citri str. 306
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PMP0 (Residues: 1-563; Coverage: 81%)
Gene names: XAC1386, metG, metS
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand U7S 1 x U7S
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 77.161Å b: 84.418Å c: 95.823Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.255
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

A Systematic Strategy to Find Potential Therapeutic Targets for Pseudomonas aeruginosa Using Integrated Computational Models.
Medeiros Filho et al. (2021)
2 more

2 mentions without citation

Molecular basis for diaryldiamine selectivity and competition with tRNA in a type 2 methionyl-tRNA synthetase from a Gram-negative bacterium.
Mercaldi et al. (2021)
1 more