6wus

X-ray diffraction
2.76Å resolution

Crystal structure of PRL-1 phosphatase C104D mutant in complex with the Bateman domain of CNNM2 magnesium transporter

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-174824 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Metal transporter CNNM2 Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.41 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3TWN3 (Residues: 430-580; Coverage: 17%)
Gene names: Acdp2, Cnnm2
Sequence domains: CBS domain
Protein tyrosine phosphatase type IVA 1 Chain: B
Molecule details ›
Chain: B
Length: 164 amino acids
Theoretical weight: 18.81 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q63739 (Residues: 7-160; Coverage: 89%)
Gene names: Prl1, Ptp4a1
Sequence domains: Polymorphic toxin system, DSP-PTPase phosphatase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CHESS BEAMLINE F1
Spacegroup: I222
Unit cell:
a: 52.138Å b: 127.935Å c: 152.158Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.24 0.281
Expression system: Escherichia coli