› 6wvf

X-ray diffraction
2.9Å resolution

E.coli DsbB C104S with ubiquinone

Released:
DOI: 10.2210/pdb6wvf/pdb
PDB entry 6wvf coloured by chain, front view.
PDB entry 6wvf coloured by chain, side view.
PDB entry 6wvf coloured by chain, top view.
Source organisms:
Primary publication:
Termini restraining of small membrane proteins enables structure determination at near-atomic resolution.
Liu S, Li S, Yang Y, Li W
Sci Adv 6 (2020)
PMID: 33355146

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-531884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disulfide bond formation protein B; Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 45.41 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-145, 146-231; Coverage: 97%)
  • Canonical: P0A6M2 (Residues: 7-167; Coverage: 92%)
Gene names: GFP, JW5182, b1185, dsbB, roxB, ycgA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand UQ1 1 x UQ1
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 53.381Å b: 53.381Å c: 280.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.259 0.286
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Highlighting membrane protein structure and function: A celebration of the Protein Data Bank.
Li et al. (2021)
3 more

2 mentions without citation

Termini restraining of small membrane proteins enables structure determination at near-atomic resolution.
Liu et al. (2020)
1 more