› 6wwc

X-ray diffraction
2.56Å resolution

Vaccine-elicited mouse FP-targeting neutralizing antibody vFP16.02 with S48K mutation in light chain in complex with HIV fusion peptide (residue 512-519)

Released:
DOI: 10.2210/pdb6wwc/pdb
PDB entry 6wwc coloured by chain, front view.
PDB entry 6wwc coloured by chain, side view.
PDB entry 6wwc coloured by chain, top view.
Source organisms:
Primary publication:
Mutational fitness landscapes reveal genetic and structural improvement pathways for a vaccine-elicited HIV-1 broadly neutralizing antibody.
Madan B, Zhang B, Xu K, Chao CW, O'Dell S, Wolfe JR, Chuang GY, Fahad AS, Geng H, Kong R, Louder MK, Nguyen TD, Rawi R, Schön A, Sheng Z, Nimrania R, Wang Y, Zhou T, Lin BC, Doria-Rose NA, Shapiro L, Kwong PD, DeKosky BJ
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33649208

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-528587 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
vFP16.02 antibody heavy chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 217 amino acids
Theoretical weight: 23.07 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
vFP16.02 antibody light chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 218 amino acids
Theoretical weight: 24.18 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Transmembrane protein gp41 Chains: C, F
Molecule details ›
Chains: C, F
Length: 8 amino acids
Theoretical weight: 733 Da
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: P04578 (Residues: 512-519; Coverage: 1%)
Gene name: env

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 68.628Å b: 70.526Å c: 93.292Å
α: 90° β: 94.79° γ: 90°
R-values:
R R work R free
0.212 0.209 0.266
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

13 citation in other articles

Binding affinity landscapes constrain the evolution of broadly neutralizing anti-influenza antibodies.
Phillips et al. (2021)
12 more