› 6wyi

X-ray diffraction
1.92Å resolution

Crystal structure of EchA19, enoyl-CoA hydratase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb6wyi/pdb
PDB entry 6wyi coloured by chain, front view.
PDB entry 6wyi coloured by chain, side view.
PDB entry 6wyi coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Post-translational Succinylation of Mycobacterium tuberculosis Enoyl-CoA Hydratase EchA19 Slows Catalytic Hydration of Cholesterol Catabolite 3-Oxo-chol-4,22-diene-24-oyl-CoA.
Bonds AC, Yuan T, Werman JM, Jang J, Lu R, Nesbitt NM, Garcia-Diaz M, Sampson NS
ACS Infect Dis 6 2214-2224 (2020)
PMID: 32649175

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA hydratase EchA19 Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 30.55 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53561 (Residues: 3-3, 5-266; Coverage: 99%)
Gene names: Rv3516, echA19
Sequence domains: Enoyl-CoA hydratase/isomerase

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P321
Unit cell:
a: 75.413Å b: 75.413Å c: 68.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.182 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Lipid Droplets, the Central Hub Integrating Cell Metabolism and the Immune System.
Zhang et al. (2021)
1 more

1 mention without citation

Post-translational Succinylation of Mycobacterium tuberculosis Enoyl-CoA Hydratase EchA19 Slows Catalytic Hydration of Cholesterol Catabolite 3-Oxo-chol-4,22-diene-24-oyl-CoA.
Bonds et al. (2020)