› 6wyl

Electron Microscopy
3.9Å resolution

Cryo-EM structure of GltPh L152C-G351C mutant in the intermediate outward-facing state.

Released:
DOI: 10.2210/pdb6wyl/pdb
PDB entry 6wyl coloured by chain, front view.
PDB entry 6wyl coloured by chain, side view.
PDB entry 6wyl coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Glutamate transporters have a chloride channel with two hydrophobic gates.
Chen I, Pant S, Wu Q, Cater RJ, Sobti M, Vandenberg RJ, Stewart AG, Tajkhorshid E, Font J, Ryan RM
Nature 591 327-331 (2021)
PMID: 33597752
Related structures: EMD-21968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate transporter homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 422 amino acids
Theoretical weight: 44.59 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59010 (Residues: 1-417; Coverage: 98%)
Gene name: PH1295
Sequence domains: Sodium:dicarboxylate symporter family

Ligands and Environments

1 bound ligand:
Ligand ASP 3 x ASP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-21968
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Regulation of Glutamate, GABA and Dopamine Transporter Uptake, Surface Mobility and Expression.
Ryan et al. (2021)
7 more

3 mentions without citation

Viral macrodomains: a structural and evolutionary assessment of the pharmacological potential.
Rack et al. (2020)
2 more