› 6wza

X-ray diffraction
2.5Å resolution

Ni-bound structure of an engineered metal-dependent protein trimer, TriCyt1

Released:
DOI: 10.2210/pdb6wza/pdb
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PDB entry 6wza coloured by chain, front view.
PDB entry 6wza coloured by chain, side view.
PDB entry 6wza coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites.
Kakkis A, Gagnon D, Esselborn J, Britt RD, Tezcan FA
Angew Chem Int Ed Engl 59 21940-21944 (2020)
PMID: 32830423

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 106 amino acids
Theoretical weight: 11.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

5 bound ligands:
Ligand HEC 3 x HEC
Ligand NI 1 x NI
Ligand NA 5 x NA
Ligand CL 6 x CL
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3121
Unit cell:
a: 82.115Å b: 82.115Å c: 137.517Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.241 0.306
Expression system: Escherichia coli

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Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
12 other articles

1 mention without citation

Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites.
Kakkis et al. (2020)

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