› 6x1v

X-ray diffraction
2.11Å resolution

Structure of pHis Fab (SC44-8) in complex with pHis mimetic peptide

Released:
DOI: 10.2210/pdb6x1v/pdb
PDB entry 6x1v coloured by chain, front view.
PDB entry 6x1v coloured by chain, side view.
PDB entry 6x1v coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for differential recognition of phosphohistidine-containing peptides by 1-pHis and 3-pHis monoclonal antibodies.
Kalagiri R, Stanfield RL, Meisenhelder J, La Clair JJ, Fuhs SR, Wilson IA, Hunter T
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 33547238

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211774 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SC44-8 Light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.01 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
SC44-8 Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 23.42 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
ACLYana-3-pTza peptide Chain: D
Molecule details ›
Chain: D
Length: 9 amino acids
Theoretical weight: 749 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 50.408Å b: 66.296Å c: 58.578Å
α: 90° β: 94.944° γ: 90°
R-values:
R R work R free
0.18 0.177 0.22
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

5 review citations

A journey from phosphotyrosine to phosphohistidine and beyond.
Hunter T. (2022)
4 more