› 6x1w

X-ray diffraction
1.95Å resolution

Structure of pHis Fab (SC56-2) in complex with pHis mimetic peptide

Released:
DOI: 10.2210/pdb6x1w/pdb
PDB entry 6x1w coloured by chain, front view.
PDB entry 6x1w coloured by chain, side view.
PDB entry 6x1w coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for differential recognition of phosphohistidine-containing peptides by 1-pHis and 3-pHis monoclonal antibodies.
Kalagiri R, Stanfield RL, Meisenhelder J, La Clair JJ, Fuhs SR, Wilson IA, Hunter T
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 33547238

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211775 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SC56-2 Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 23.54 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
SC56-2 Light chain Chain: L
Molecule details ›
Chain: L
Length: 221 amino acids
Theoretical weight: 23.58 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
ACLYana-3-pTza peptide Chain: A
Molecule details ›
Chain: A
Length: 9 amino acids
Theoretical weight: 749 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P41212
Unit cell:
a: 121.06Å b: 121.06Å c: 77.956Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.241
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

5 review citations

A journey from phosphotyrosine to phosphohistidine and beyond.
Hunter T. (2022)
4 more