› 6x3j

X-ray diffraction
2.7Å resolution

Crystal structure of streptogramin A acetyltransferase VatA from Staphylococcus aureus in complex with streptogramin analog F0224 (46)

Released:
DOI: 10.2210/pdb6x3j/pdb
PDB entry 6x3j coloured by chain, front view.
PDB entry 6x3j coloured by chain, side view.
PDB entry 6x3j coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Synthetic group A streptogramin antibiotics that overcome Vat resistance.
Li Q, Pellegrino J, Lee DJ, Tran AA, Chaires HA, Wang R, Park JE, Ji K, Chow D, Zhang N, Brilot AF, Biel JT, van Zundert G, Borrelli K, Shinabarger D, Wolfe C, Murray B, Jacobson MP, Mühle E, Chesneau O, Fraser JS, Seiple IB
OpenAccess logo Nature 586 145-150 (2020)
PMID: 32968273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Virginiamycin A acetyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 209 amino acids
Theoretical weight: 23.81 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P26839 (Residues: 7-215; Coverage: 95%)
Gene name: vat
Sequence domains: Bacterial transferase hexapeptide (six repeats)

Ligands and Environments

5 bound ligands:
Ligand O7V 6 x O7V
Ligand PO4 2 x PO4
Ligand SXA 6 x SXA
Ligand CL 11 x CL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 104.8Å b: 109.22Å c: 173.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.271
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Molecular mechanisms of antibiotic resistance revisited.
Darby et al. (2023)
3 more