› 6x68

Electron Microscopy
3.66Å resolution

Cryo-EM structure of piggyBac transposase synaptic complex with hairpin DNA (SNHP)

Released:
DOI: 10.2210/pdb6x68/pdb
PDB entry 6x68 coloured by chain, front view.
PDB entry 6x68 coloured by chain, side view.
PDB entry 6x68 coloured by chain, top view.
Source organism: Trichoplusia ni
Primary publication:
Structural basis of seamless excision and specific targeting by piggyBac transposase
Chen Q, Luo W, Veach RA, Hickman AB, Wilson MH, Dyda F
Nat Commun 11 3446-3446 (2020)
Related structures: EMD-22073

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120907 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
PiggyBac transposable element-derived protein domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 594 amino acids
Theoretical weight: 68.01 KDa
Source organism: Trichoplusia ni
Expression system: Homo sapiens
UniProt:
  • Canonical: Q283G1 (Residues: 1-594; Coverage: 100%)
Sequence domains: Transposase IS4
hairpin DNA Chains: A, B
Molecule details ›
Chains: A, B
Length: 74 nucleotides
Theoretical weight: 22.81 KDa
Source organism: Trichoplusia ni
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.66Å
Relevant EMDB volumes: EMD-22073
Expression systems:
  • Homo sapiens
  • Not provided

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