› 6xet

X-ray diffraction
2.6Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-135916 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 438 amino acids
Theoretical weight: 48.78 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-438; Coverage: 97%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 433 amino acids
Theoretical weight: 48.65 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-433; Coverage: 97%)
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 143 amino acids
Theoretical weight: 16.85 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family

Ligands and Environments

5 bound ligands:
Ligand GTP 3 x GTP
Ligand SO4 3 x SO4
Ligand GDP 1 x GDP
Ligand TU2 2 x TU2
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 66.097Å b: 126.938Å c: 250.461Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.231
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Recent Advances of Tubulin Inhibitors Targeting the Colchicine Binding Site for Cancer Therapy.
Hawash M. (2022)
1 more

1 mention without citation

Design, Synthesis, and Biological Evaluation of Stable Colchicine-Binding Site Tubulin Inhibitors 6-Aryl-2-benzoyl-pyridines as Potential Anticancer Agents.
Chen et al. (2021)