› 6xgc

Electron Microscopy
4.1Å resolution

CryoEM structure of influenza hemagglutinin A/Michigan/45/2015 in complex with cyno antibody 1C4

Released:
DOI: 10.2210/pdb6xgc/pdb
PDB entry 6xgc coloured by chain, front view.
PDB entry 6xgc coloured by chain, side view.
PDB entry 6xgc coloured by chain, top view.
Source organisms:
Primary publication:
Broad neutralization of H1 and H3 viruses by adjuvanted influenza HA stem vaccines in nonhuman primates.
Darricarrère N, Qiu Y, Kanekiyo M, Creanga A, Gillespie RA, Moin SM, Saleh J, Sancho J, Chou TH, Zhou Y, Zhang R, Dai S, Moody A, Saunders KO, Crank MC, Mascola JR, Graham BS, Wei CJ, Nabel GJ
Sci Transl Med 13 (2021)
PMID: 33658355
Related structures: EMD-22180

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-207342 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 327 amino acids
Theoretical weight: 36.43 KDa
Source organism: Influenza A virus (A/Michigan/45/2015(H1N1))
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A1J0CXU0 (Residues: 18-344; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin HA2 chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 220 amino acids
Theoretical weight: 25.11 KDa
Source organism: Influenza A virus (A/Michigan/45/2015(H1N1))
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A109YUH3 (Residues: 345-518; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
1C4 Fab heavy chain Chains: H, I, M
Molecule details ›
Chains: H, I, M
Length: 244 amino acids
Theoretical weight: 25.61 KDa
Source organism: Macaca fascicularis
Expression system: Homo sapiens
1C4 Fab light chain Chains: J, L, N
Molecule details ›
Chains: J, L, N
Length: 216 amino acids
Theoretical weight: 22.84 KDa
Source organism: Macaca fascicularis
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 15 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-22180
Expression system: Homo sapiens

Quick links

Citations

15 review citations

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
14 more