› 6xgt

X-ray diffraction
2.2Å resolution

Crystal structure of cyanase from the thermophilic fungus Thermomyces lanuginosus

Released:
DOI: 10.2210/pdb6xgt/pdb
PDB entry 6xgt coloured by chain, front view.
PDB entry 6xgt coloured by chain, side view.
PDB entry 6xgt coloured by chain, top view.
Source organism: Thermomyces lanuginosus
Primary publication:
Crystal structure of a thermophilic fungal cyanase and its implications on the catalytic mechanism for bioremediation.
Ranjan B, Choi PH, Pillai S, Permaul K, Tong L, Singh S
OpenAccess logo Sci Rep 11 277 (2021)
PMID: 33431973

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167769 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyanate hydratase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 181 amino acids
Theoretical weight: 20.11 KDa
Source organism: Thermomyces lanuginosus
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand FMT 4 x FMT
Ligand MLI 3 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 75.368Å b: 157.639Å c: 163.871Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.154 0.202
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Diversity, prevalence, and expression of cyanase genes (cynS) in planktonic marine microorganisms.
Mao et al. (2022)

1 mention without citation

Crystal structure of a thermophilic fungal cyanase and its implications on the catalytic mechanism for bioremediation.
Ranjan et al. (2021)