› 6xio

X-ray diffraction
3.12Å resolution

ADP-dependent kinase complex with fructose-6-phosphate and ADPbetaS

Released:
DOI: 10.2210/pdb6xio/pdb
PDB entry 6xio coloured by chain, front view.
PDB entry 6xio coloured by chain, side view.
PDB entry 6xio coloured by chain, top view.
Source organism: Methanosarcinales archaeon
Primary publication:
Structure of an ancestral ADP-dependent kinase with fructose-6P reveals key residues for binding, catalysis, and ligand-induced conformational changes.
Muñoz SM, Castro-Fernandez V, Guixé V
OpenAccess logo J Biol Chem 296 100219
PMID: 33839685

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-dependent phosphofructokinase Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 57.77 KDa
Source organism: Methanosarcinales archaeon
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand AT4 1 x AT4
Ligand F6P 1 x F6P
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 67.574Å b: 79.589Å c: 108.574Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.241 0.272
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Structure of an ancestral ADP-dependent kinase with fructose-6P reveals key residues for binding, catalysis, and ligand-induced conformational changes.
Muñoz et al. (2021)